Regulatory
vha-6

Part:BBa_K5233000:Design

Designed by: Yunfeng Bai   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-23)


vha-6 promoter which is a specific promoter that only expressed in the gut of C.elegans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 776
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 776
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 776
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 303


Design Notes

vha-6 is a gut specific promoter. It may not be expressed outside the gut. Also the promoter is unidirectional, so it must be located at the upstream of target gene.


Source

vha-6 comes from the genomic sequence of C.elegans,and the genomic sequence is obtained in NCBI.

References